منابع مشابه
The reactive site of trypsin inhibitors.
Virgin soybean trypsin inhibitor has 1 NHp-terminal aspartic acid or asparagine residue and no COOH-terminal residues that can be released by carboxypeptidase B. Upon incubation with 1 mole % of trypsin for 24 hours at pH 3.75, virgin inhibitor is converted to a modified inhibitor which has 2 NH2 terminals aspartic acid or asparagine and isoleucine. Approximately 1 mole of arginine is released ...
متن کاملThe Basic Trypsin Inhibitor of Bovine Pancreas IX. LOCATION OF THE REACTIVE SITE IN THE CARBOXAMIDOMETHYL
A derivative of the basic pancreatic inhibitor which contained carboxamidomethyl groups on half-cystine residues 14 and 38 was incubated with trypsin and intact inhibitor at pH 5.5. The carboxamidomethyl derivative after separation from trypsin and intact inhibitor released only lysine when incubated with carboxypeptidase B. The derivative was oxidized with performic acid and a peptide correspo...
متن کاملCompetition among trypsin inhibitors.
The experiments presented in this paper were designed to elucidate the kinetics of action of three protein inhibitors (from pancreas, soy bean, and egg white) on trypsin, and to discover how the inhibition by these substances was related to the action of diisopropyl fluorophosphate (DFP), mercuric chloride, and calcium salts on trypsin. The most, detailed work on the protein inhibitors of tryps...
متن کاملSignificance of Soya Trypsin Inhibitors
Although recent evidence clearly indicates that trypsin inhibitors (TO and low protein digestibility are the major factors responsible for the pancreatic hypertrophic and growth inhibitory effects of raw soybeans, there was uncertainty regarding the biological threshold level of TI at which these biological effects occur. To obtain such data, dehulled defatted flakes (10% dietary protein) conta...
متن کاملAnalysis by phage display selection and site-directed retromutagenesis of the Mustard Trypsin Inhibitor 2 reactive site.
The Mustard Trypsin Inhibitor (MSI) family is a small family of plant protease inhibitors so far only found in Brassicaceae. Using a phage display selection, MTI-2 (Mustard Trypsin Inhibitor 2) mutants were detected and analysed for their biochemical characteristics. Retromutants of the selected MTI-2 proteins were constructed and expressed in the Pichia pastoris system. The recombinant protein...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1966
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)99798-x